FIGURE 2.

Northern blot analysis of Os9BGlu31 gene expression in rice cv. Yukihikari plants and seedlings. A, blot of RNA extracted from various tissues of 6-week-old mature plants and 10-day-old rice seedlings. B, total RNA was extracted from whole seeds or seedlings germinated and grown at 27 °C for the indicated periods of time. C, effects of stresses and phytohormones on Os9BGlu31 gene expression in 10-day-old rice seedlings. RNA gel blots of 10-day-old rice seedlings treated for an additional 2 days with the indicated stresses or phytohormones: H₂O (control), cold (5 °C and 12 °C), drought, 0.3 m NaCl, flooding, 0.1 mm ABA, 10 mm ethephon, 0.1 mm methyljasmonate, 0.1 mm gibberellin A₃ (GA₃), 10 mm 2,4-D, and 0.1 mm kinetin. D, effects of ethephon on different tissues of 10-day-old rice seedlings. As in C, 10-day-old rice seedlings were treated an additional 2 days with water (control) or 10 mm ethephon.