TABLE 3.

Apparent kinetic parameters of Os9BGlu31with aromatic acid acceptors

Acceptor	Kinetic parameters for acceptor at 30 mm 4NPGlc			Kinetic parameters for 4NPGlc at 0.25 mm acceptor
	Km	kcat	kcat/Km	Km	kcat	kcat/Km
	mm	s−1	mm−1 s−1	mm	s−1	mm−1 s−1
Phenolic compounds
Ferulic acid	0.05 ± 0.004	1.21 ± 0.06	25.42 ± 1.52	9.33 ± 0.62	1.21 ± 0.2	0.13 ± 0.01
Vanillic acid	0.11 ± 0.012	0.43 ± 0.04	3.82 ± 0.4	5.92 ± 0.28	0.36 ± 0.023	0.06 ± 0.005
4-Hydroxybenzoic acid	0.21 ± 0.02	0.49 ± 0.002	2.31 ± 0.22	2.88 ± 0.27	0.33 ± 0.01	0.21 ± 0.01
Syringic acid	0.13 ± 0.012	0.35 ± 0.03	2.70 ± 0.25	1.18 ± 0.13	0.24 ± 0.02	0.20 ± 0.02
trans-Cinnamic acid	0.10 ± 0.01	0.32 ± 0.02	3.26 ± 0.08	1.62 ± 0.17	0.12 ± 0.004	0.07 ± 0.002
Caffeic acid	0.03 ± 0.003	0.25 ± 0.005	7.62 ± 0.6	7.70 ± 0.6	0.25 ± 0.02	0.03 ± 0.0004
Sinapic acid	0.02 ± 0.002	0.29 ± 0.01	14.15 ± 0.4	7.06 ± 0.74	0.28 ± 0.02	0.05 ± 0.001
Benzoic acid	0.12 ± 0.014	0.35 ± 0.038	2.87 ± 0.5	2.00 ± 0.17	0.28 ± 0.02	0.14 ± 0.04
4-Coumaric acid	0.01 ± 0.001	0.34 ± 0.03	33.3 ± 3.9	3.31 ± 0.3	0.30 ± 0.03	0.10 ± 0.008
Isovanillic acid	0.042 ± 0.003	0.22 ± 0.001	5.13 ± 0.5	1.91 ± 0.2	0.17 ± 0.02	0.10 ± 0.007
Dihydroxybenzoic acid	0.05 ± 0.004	0.12 ± 0.01	2.59 ± 0.2	0.64 ± 0.006	0.12 ± 0.01	0.19 ± 0.02
Phytohormones
Napthalene acetic acid	0.09 ± 0.007	0.12 ± 0.003	1.46 ± 0.02	0.27 ± 0.03	0.11 ± 0.001	0.39 ± 0.034
Indole acetic acid	0.05 ± 0.005	0.09 ± 0.001	2.05 ± 0.21	0.33 ± 0.02	0.1 ± 0.002	0.31 ± 0.01