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. 2013 Feb 20;288(14):10144–10153. doi: 10.1074/jbc.M113.451526

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Residue 25 is responsible for the difference in acyl-CoA preference of ssSPTa and ssSPTb. Total LCBs prepared from lcb1Δlcb2Δtsc3Δ mutant yeast expressing hLCB1, hLCB2a and the indicated wild-type or mutant ssSPT subunit were analyzed by HPLC as described under “Experimental Procedures.” A and B, complete elution profiles containing all of the C18 and C20 LCBs. C–F, only the regions of the elution profiles containing C18-dihydrosphinganine (DHS), C20-PHS, and C18-anhydrous PHS since the C20-PHS, eluting at 22.5 min, is diagnostic for the presence of an active ssSPTb subunit.