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In vitro SPT activity and acyl-CoA selectivity of ssSPT residue 25 mutants. A, microsomes prepared from lcb1Δlcb2Δtsc3Δ mutant yeast cells expressing hLCB1, hLCB2a, and wild-type or mutant ssSPTs were assayed for SPT activity using either C16- or C18-CoA as described in Fig. 3. B, microsomes prepared from cells expressing hLCB1, hLCB2a, and the indicated ssSPTa mutants were assayed for SPT activity using C14-, C16-, C18-, C20- or C22-CoA as substrate.
