FIGURE 9.
A disease-causing mutation in hLCB1 results in decreased activation by ssSPTa but not by ssSPTb. A, the lcb1Δlcb2Δtsc3Δ mutant yeast cells expressing hLCB2a and wild-type or mutant (S331F) hLCB1 were grown on YPD medium at 26 °C or 37 °C, or on SD medium at 26 °C with PHS. B, yeast cells expressing triple fusion SPTs containing the indicated ssSPT were grown as in A. C, LCBs were prepared from yeast expressing the indicated triple fusion SPTs and analyzed by HPLC as in Fig. 8. D, microsomes were prepared from yeast expressing the indicated wild-type hLCB1- and hLCB1S331F-containing fusions, and SPT activity was measured using palmitoyl-CoA as described above.