FIGURE 2.
Hdac3 regulates chondrocyte hypertrophy and matrix gene expression. A, paraffin-embedded tibia sections were obtained from P1 WT or Hdac3-CKOOsx mice, stained with Alcian blue, and photographed (4×). The arrows indicate length measurements shown in B. B, the distance from the bone epiphysis to the onset of the hypertrophic zone was determined. C–E, total RNA was extracted from WT or Hdac3-CKOOsx P1 tibias. Relative expression of matrix genes, including aggrecan (C), osteopontin (D), and MEPE (E), was determined by real-time PCR. *, p < 0.05.