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. 2013 Feb 13;288(14):9572–9582. doi: 10.1074/jbc.M112.423723

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Hdac3 regulates chondrocyte cell size. A and B, distal femurs from 5-week-old Cre-negative (Hdac3^fl^/+;WT Cre⁻), Cre-positive (Osx-Cre⁺:Hdac3^+/+;WT Cre⁺), and Osx-driven CKO (Osx-Cre⁺:Hdac3^fl/fl; Hdac3-CKO_Osx) mice were stained with Goldner's Trichrome (A) or Alcian blue (B). C, the angles between three adjacent columnar chondrocytes were assessed using ImageJ software. Representative tracings from >50 are shown in A. D, the average hypertrophic chondrocyte cell perimeter (n = 200) was determined using ImageJ software. *, p < 0.05 compared with WT.