FIGURE 2.

The RING domain of Bre1p promotes the association of RNA polymerase II with GAL1. A, schematic diagram of the Bre1p mutant without the RING domain. B, analysis of RNA polymerase II association with GAL1 in the presence and absence of the RING domain of Bre1p. Yeast strains (RSY12 and RSY13) were grown, cross-linked, and immunoprecipitated as described in the legend to Fig. 1B. C, analysis of GAL1 transcription by RT-PCR. Yeast cells were grown as described in the legend to Fig. 1B. D, Western blot analysis.