FIGURE 5.

A non-RING domain of Bre1p represses the association of RNA polymerase II with GAL1. A, schematic diagram of different Bre1p mutants. B, Western blot analysis. C, analysis of RNA polymerase II association with GAL1 in different Bre1p mutants. Yeast strains (RSY12, RSY13, RSY14, and RSY15) were grown, cross-linked, and immunoprecipitated as described in the legend to Fig. 1B. The p values for the differences between wild-type and Bre1p mutants are presented on top of the histograms of the mutants. D, analysis of GAL1 transcription by RT-PCR. Yeast cells were grown as described in the legend to Fig. 1B.