FIGURE 6.

Analysis of the association of RNA polymerase II with GAL10. A, a schematic diagram showing the locations of the primer pairs (ORF1 and ORF2) at the GAL10 coding sequence for ChIP analysis. The numbers are presented with respect to the position of the first nucleotide of the initiation codon (+1). B, Rad6p promotes the association of RNA polymerase II with GAL10. Yeast strains (STY1 and STY2) were grown, cross-linked, and immunoprecipitated as described in the legend to Fig. 1B. C, association of RNA polymerase II with GAL10 is significantly decreased in the histone H2B-K123R point mutant strain. Yeast strains (YKH045 and YKH046) were grown, cross-linked, and immunoprecipitated as described in the legend to Fig. 1B. D, association of RNA polymerase II with GAL10 is not impaired in the absence of Bre1p. Yeast strains (YTT31 and SLY1a) were grown, cross-linked, and immunoprecipitated as described in the legend to Fig. 1B. E, analysis of GAL10 transcription by RT-PCR. Yeast strains were grown as described in the legend to Fig. 1B prior to harvesting for mRNA analysis. F and G, analysis of RNA polymerase II association with GAL10 and transcription in the bre1Δ200 and wild-type strains. Both wild-type and mutant strains were grown as described in the legend to Fig. 1B.