TABLE 2.
Properties of mIPSCs recorded
Transfected neurons were used at DIV 11 + 4 for recording the mIPSCs as described under “Experimental Procedures.” The values are given as the means ± S.E. Statistical analysis was done using unpaired Student's t test and ANOVA. Neurons were treated with vehicle (saline) or PD98059 (25 μm) for 3 h at DIV 15 prior to recording the mIPSC currents.
| Events | Amplitude | Interevent interval | Rise time | Decay time | |
|---|---|---|---|---|---|
| pA | ms | ms | ms | ||
| In hippocampal neurons transfected with eGFP-gephyrin constructs | |||||
| WT | 329 | 36.8 ± 0.7 | 2181 ± 143 | 4.1 ± 0.3 | 22.9 ± 1.2 |
| Mock | 240 | 38.4 ± 0.8 | 1996 ± 129 | ||
| S268A | 479 | 49.3 ± 0.7a,c | 1198 ± 64a,b | 3.9 ± 0.3 | 22.5 ± 0.79 |
| S268E | 161 | 40.3 ± 0.8a | 2079 ± 222 | 3.4 ± 0.4 | 21.7 ± 1.1 |
| In hippocampal neurons treated with PD98059 | |||||
| Vehicle | 1599 | 47.4 ± 0.8 | 860 ± 28 | ||
| PD98059 | 1250 | 60.6 ± 1.1c | 1353 ± 50c |
a p < 0.001 between S268A and S268E.
b p < 0.001 between WT and S268A or S268E.
c p < 0.001, unpaired Student's t test.