FIGURE 4.

The MYND-type zinc finger of PHD2 is required for its interaction with p23. A and B, HEK293FT were transfected and immunoprecipitated (IP) as in Fig. 1B. WB, Western blot. C, shown is a schematic diagram of PHD2. Dark shading denotes the N-terminal MYND-type zinc finger (ZF), light shading denotes the C-terminal prolyl hydroxylase (PH) domain, and numbers indicate amino acid number. Shown at the top are amino acids 21–58. Underlining indicates predicted zinc chelating residues. Inverted triangles indicate Cys-36 and Cys-42.