FIGURE 5.

Knockdown of p23 augments HIF-1α protein levels. A, the indicated cell lines were treated with the indicated siRNA and then maintained under normoxia (NX) or subjected to 2% O₂ (HX) for 4 h. Cell lysates were prepared, and equal protein amounts were then subjected to Western blotting using antibodies to the indicated proteins. Con = control non-targeting siRNA #3. The p23 siRNA was PTGES3_1. B, HeLa cells were treated with the indicated siRNA and then examined as in A. The p23-1 siRNA was PTGES3_1, and the p23-2 siRNA was PTGES3_2.