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. 2013 Feb 19;288(14):9729–9741. doi: 10.1074/jbc.M113.453266

FIGURE 6.

FIGURE 6.

Substoichiometric concentrations of either LBP or soluble CD14 are sufficient to enhance TLR1·TLR2·Pam3CSK4 ternary complex formation. 0.25 μm TLR1, 0.25 μm TLR2, and 2.5 μm Pam3CSK4 were incubated with various concentrations (250, 50, 10, 5, and 0 nm) of either LBP (left panel) or sCD14 (right panel) for 2 h in a 500-μl volume at 37 °C in PBS buffer, pH 7.4. Proteins and protein complexes were separated and analyzed as described in the legend of Fig. 2. The results shown are representative of at least three independent experiments. mAu, milliabsorbance units.