FIGURE 6.

Substoichiometric concentrations of either LBP or soluble CD14 are sufficient to enhance TLR1·TLR2·Pam₃CSK₄ ternary complex formation. 0.25 μm TLR1, 0.25 μm TLR2, and 2.5 μm Pam₃CSK₄ were incubated with various concentrations (250, 50, 10, 5, and 0 nm) of either LBP (left panel) or sCD14 (right panel) for 2 h in a 500-μl volume at 37 °C in PBS buffer, pH 7.4. Proteins and protein complexes were separated and analyzed as described in the legend of Fig. 2. The results shown are representative of at least three independent experiments. mAu, milliabsorbance units.