TABLE 1.

The sequences of the PCR primers used to obtain the 7R mutants

Mutated codons are in bold. TSM, triple substitution mutant; GZMD, glycine zipper motif (amino acids 189–197) deletion mutant.

Mutants	Primer name	Primer sequence (5′–3′)
SSM/S185L	m7R 4F	CAGACCTCCTTCCTCCTTAACGTCTCTGGCCTT
	m7R 4R	AAGGCCAGAGACGTTAAGGAGGAAGGAGGTCTG
SSM/G189L	m7R 5F	CTCAGTAACGTCTCTCTCCTTTTGATTGGCCTG
	m7R 5R	CAGGCCAATCAAAAGGAGAGAGACGTTACTGAG
SSM/G193L	m7R 6F	CTCTGGCCTTTTGATTCTCCTGTCCTATGGCCT
	m7R 6R	AGGCCATAGGACAGGAGAATCAAAAGGCCAGAG
SSM/G197L	m7R 7F	GATTGGCCTGTCCTATCTCCTCACCTACTGCTAC
	m7R 7R	GTAGCAGTAGGTGAGGAGATAGGACAGGCCAATC
TSM	m7R 8F	CTCTCTCCTTTTGATTCTCCTGTCCTATCTCCT
	m7R 8R	AGGAGATAGGACAGGAGAATCAAAAGGAGAGAG
GZMD	m7R 9F	AACGTCTCTCTCACCTACTGCTACTCCCTCGACCTC
	m7R 9R	GTAGGTGAGAGAGACGTTACTGAGGAAGGAGGTCTG