The effect of phospho-tailpiece peptides on IIC-Rod1296–1854 filament assembly.
A, 5 μm IIC-Rod1296–1854 was incubated in the presence of 20 μm tailpiece peptides and subjected to filament assembly assay as described in the legend for Fig. 2. S, supernatant; P, pellet. B, CD spectra of IIC-Rod1296–1854 with the phospho-tailpiece peptides. Black line, CD spectra of 0.01 mm IIC-Rod1296–1854 alone; blue line, with Tailpiece1946–1967; red line, Tailpiece1968–2000; magenta line, Tailpiece1946–1967-pT1957; cyan line, with Tailpiece1946–1967-pT1960.