TABLE 1.
Comparison of anti-CD9 mAb, SCF, and Ag in their ability to induce signaling events in mast cells
| Parametera | Anti-CD9 | SCF | Ag |
|---|---|---|---|
| Protein phosphorylation | |||
| Akt-S473 | −b | +++ | +++ |
| Akt-T308 | − | +++ | +++ |
| Erk-Y204 | + | +++ | +++ |
| p38-T180/Y182 | + | +++ | +++ |
| Syk | − | − | +++ |
| NTAL | ++ | + | +++ |
| LAT | −/+ | − | +++ |
| Protein dephosphorylation | |||
| ERM-T567/564/558 | ++ | ++ | ++ |
| Degranulation (β-glucuronidase) | + | + | +++ |
| Ca2+ mobilization | + | + | +++ |
| Adhesion | − | ++c | ++ |
| Spreading | − | +++c | +++ |
| Chemotaxis | − | +++c | ++ |
a Specific protein phosphorylation or dephosphorylation, degranulation, Ca2+ mobilization, adhesion, and chemotactic potential of CD9-specific mAb 2H9, SCF and Ag were measured using appropriate methods as indicated under ”Experimental Procedures“ and ”Results.“
b −, No signal; +, weak signal; ++, medium signal; +++, strong signal.
c See Ref. 14.