FIGURE 1.

Endogenous JAK3, FES, and PLD2 activity levels in untransformed and transformed cells. JAK3 (A), Fes (B), and PLD2 (C) activities were measured in unstimulated and untransfected MCF10A and MDA-MB-231 cell lysates. The experiments started from similar number of cells per sample and then adjusted for similar protein levels per sample, so proper comparison can be made. Results in this figure are the means + S.E. from at least three independent experiments conducted in duplicate. The asterisk denotes statistically significant (p < 0.05) differences (increases) between samples and controls. Western blot (W.B.) analysis of endogenous JAK3 (D), Fes (E), and PLD2 (F) proteins (100 μg/lane, similar amount for each cell type) in non-transformed MCF10A and transformed MDA-MB-231 cancer cell lines with actin expression included as equal protein loading controls.