FIGURE 6.

IFN-γ induces ACSL1 expression partly through JNK. A, IFN-γ treatment significantly induced ACSL1 expression in macrophages. B, Acsl1 expression was diminished in Mapk8^−/−Mapk9^−/− macrophages subsequent to treatment with IFN-γ. C, robust increases in STAT1 phosphorylation were evident 2 h after macrophage stimulation with LPS or E. coli. D, macrophage transfection with Stat1-targeting siRNA generated >70% knockdown of STAT1 protein (basal and LPS data derive from two different blots, but control siRNA and Stat1 siRNA lanes are from the same blot for basal and LPS-stimulated samples, respectively, and β-actin blots for control siRNA-treated samples are the same as in Fig. 5E because these samples were run on the same gels). However, STAT1 knockdown did not reduce LPS-stimulated Acsl1 expression (E). **, p < 0.01 versus control siRNA; ***, p < 0.001 versus basal; ###, p < 0.001 versus IFN-γ-stimulated WT. Error bars, S.E.