FIGURE 7.

ACSL1 deficiency leads to reduced 16:0-CoA, 18:1-CoA, and 20:4-CoA levels in LPS-stimulated macrophages but does not affect ceramide levels. ACSL1-deficient BMDMs and WT controls were stimulated with LPS or vehicle for 24 h, and saturated and unsaturated acyl-CoA species were measured by LC-ESI-MS/MS (A and B). Ceramides were measured by direct infusion ESI-MS, as described in detail under “Experimental Procedures.” C, ceramide molecular species in ACSL1-deficient BMDMs and WT controls stimulated with LPS for 12 h. *, p < 0.05; **, p < 0.01; ***, p < 0.001 versus the indicated treatment group; white bars, WT macrophages; gray bars, ACSL1-deficient macrophages. Error bars, S.E.