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Overview of mass defect classification of glycopeptides. Optional, initial glycopeptide enrichment is followed by LC-MS or LC-MS/MS analysis. After peak picking and deconvolution, a list of monoisotopic m/z values and retention times is generated. This list is then sorted into likely glycopeptide and likely peptide precursors on the basis of accurate mass. Targeted LC-MS/MS analysis is then possible without prior proteomic or glycomic characterization.
