Fig. 2.

a and b Pre-treatment with A-amanitin promotes significant increase of versican mRNA 3 h after E2 administration, strongly suggesting an increase in mRNAm stability. Otherwise, 12 h after 0.1 μg E2 administration there is a significant decrease in versican mRNA levels (3 vs 12 h), and 12 h after 10 μg E2 injection versican transcript content remains unchanged, when compared to the control group, indicating that mRNA stability is no longer enhanced. The decaying curve observed shows that transcription blocking was effective. Multiple comparisons performed by one-way analysis of variance (ANOVA) followed by the Student–Newman–Keuls post hoc test. Values represent the mean ± SEM on five independent tissue preparations. *p < 0.05; **p < 0.01