Fig. 4.

a In the control group (vehicle) a faint immunoreaction is observed in the subepithelial region of the antimesometrial stroma (asterisk); b 30 min; c 1 h and d 3 h after E2 injection, immunoreaction is similar to the control group; e 6 h; f 12 h and g 24 h after treatment there is a strong immunoreaction in the whole endometrial stroma and in the connective tissue between muscle layers; h and i show the positive and negative control, respectively. After a-amanitin treatment, immunoreaction for versican is present only in the superficial stroma in j control group. In k 3 h after treatment, a moderate immunoreaction is observed in the whole endometrial stroma; l In the 12 h group, the staining is restricted to the superficial stroma. L uterine lumen, SS Superficial Stroma, DS Deep Stroma, M Myometrium. Scale Bar = 200 μm