Figure S2.

TOR/InR/Pi3K Signaling Promotes NE Expansion during Early Larval Stages, Related to Results

(A) Simplified schematic representation of the TOR/InR/Pi3K network. In cells, amino-acid sensors respond to dietary nutrients by activating the TOR kinase, which phosphorylate 4E-BP to promote RNA translation. Organismal growth is regulated by Ilps that bind the InR to activate Pi3K and Akt1. Both pathways converge in promoting cell growth. The TOR kinase is a central node for nutrient sensing and cell growth activation.

(B) The NE of Tor^ΔP mutant larvae fails to initiate proliferation. In Akt1¹ mutants, NE expansion is also severely affected. E-cad (green), Mira (red), PH3 (white).

(C) In the expanding NE of 50 hr larvae, 4E-BP is strongly phosphorylated. However, if the larvae is transferred to NR conditions for 24 hr, phosphorylated 4E-BP becomes undetectable in the NE, while still present in some central brain PH3+ neuroblasts (yellow arrows). The medulla is delineated by yellow dashed line. E-Cadherin and PH3 (green), p-4E-BP (red), Mira (blue).