Figure S1.

Succination of Protein Targets in Fh1KO MEFs, Related to Table 1 and Figure 1

(A) Mitochondrial fraction of Fh1WT, Fh1KO, Fh1+FH and Fh1+FHcyt MEFs with immunoblotting for respective subcellular markers, FH and 2SC.

(B) Pie chart showing the diversity and relative proportions of biological processes encompassing proteins identified as 2SC targets. Chart created using PANTHER (Protein Analysis Through Evolutionary Relationships) (http://www.pantherdb.org/).

(C) MS/MS spectrum showing succination at both C448 and C451 in the ₄₃₈-DVGGIVLANA(^2SC)GP(^2SC)IGQWDR-457 peptide derived from human ACO2 expressed stably in Fh1KO MEFs. Selected fragments were assigned as follows: b: N-terminal fragment ion; y: C-terminal fragment ion; ^∗: fragment ion minus NH₃; 0: fragment ion minus H₂O; +: singly charged fragment ion and ++: doubly charged fragment ion; PE: pyridylethylated; 2S:succinated. Both theoretical mass (in brackets) and detected mass are given for each assigned fragment ion.