Cell-Surface Analysis of Deficient Galactosylation and Increased Terminal β-GlcNAc and α-GalNAc
(A) Flow cytometry analysis of Alexa-647-conjugated GSII (Griffonia simplicifolia) lectin in control and individual fibroblasts showing increased lectin binding in all three individuals. The parallel control is representative of three different lines that were tested.
(B) Flow cytometry analysis of fluorescein-labeled VVA (Vicia Villosa) lectin in control and individual fibroblasts also showing increased binding in all three individuals. The parallel control is representative of three different lines that were tested.