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. 2013 Apr 2;104(7):1401–1409. doi: 10.1016/j.bpj.2013.02.044

Figure 3.

Figure 3

Theoretically computed intensity in the BFP of a 1.49 NA objective, as produced by a fluorophore modeled as a single oscillating dipole on a glass coverslip centered on the optical axis and oriented parallel to the coverslip surface in the x direction, calculated according to the methods Axelrod (8). The border between subcritical (radii corresponding to 0–1.33 NA) and supercritical (radii corresponding to 1.33–1.49 NA) emission features a sudden jump in intensity.