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Scheme of the MAC. The filamentous biotinylated (blue) actin is coupled via neutravidin to the free-standing membrane (Egg PC) containing biotinylated lipids (DSPE-PEG(2000)-Biotin). For a more compact display the binding of actin is shown only to the upper leaflet of the membrane. In the experiments, actin presumably binds to both leaflets, because both membrane sides are accessible and contain biotinylated lipids.
