Figure 8.

Addition of myosin II allows switching the diffusive state of the membrane. The left image shows the fluorescence of actin (labeled with phalloidin-Alexa488) linked to membranes. Upon addition of myosin II, the actin filaments retract from most of the free-standing membrane patches, but also condense on some spots as shown in the right image. The diffusive behavior is modified accordingly. On the spots where actin was removed, the diffusion is comparable to the diffusion in a membrane before actin coupling, whereas the diffusion is reduced at spots where actin was concentrated. Both measurements are for the labeled lipid (Atto647N-DOPE). Scale bars 5 μm.