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. 2013 Apr 2;104(7):1517–1528. doi: 10.1016/j.bpj.2013.02.026

Figure 2.

Figure 2

GTP-γ-S makes microtubules more flexible. (A) Histograms of data after log-transforming the persistence length show no difference between Taxol-stabilized microtubules (red bars) and microtubules polymerized in the presence of GTP-γ-S with Taxol (purple bars) and without Taxol present (magenta bars). We fit the data to Gaussian functions (Eq. 2); fit parameters are given in Table S1. (B) Normalized cumulative distributions of persistence length for Taxol-stabilized microtubules (red circles) and microtubules polymerized in the presence of GTP-γ-S with Taxol (purple squares) or without Taxol (magenta diamonds) show overlapping distributions, with a slight shift toward lower persistence lengths for both GTP-γ-S microtubule data. (C) We see no length dependence of the persistence lengths for Taxol-stabilized microtubules (red circles) or for microtubules polymerized in the presence of GTP-γ-S with Taxol (purple squares) or without Taxol (magenta diamonds). Error bars represent the mean ± SE. The numbers of microtubules in each distribution are given in Table 1.