FIG 3.

Alkylglycerol monooxygenase topography in the lipid bilayer. Nine transmembrane (TM) and one membrane associated domain have been predicted for the alkylglycerol monooxygenase sequence. Length and positioning of the first five was further refined by ab initio modeling using Rosetta membrane (number of starting and end residue of each helix is annotated). Mutations introduced by site directed mutagenesis are shown as symbols: upward directed triangles, eight histidines motif; downward directed triangles, other histidines; circles, glutamates and aspartates; squares, all other mutated residues. Mutations shown with symbols with white lining and black filling all completely abolished alkylglycerol monooxygenase activity. The glutamate at position 137 which has been shown to interact with tetrahydrobiopterin is shown as a hexagon. The potential active site of the enzyme is highlighted in grey. Reproduced with permission, from Watschinger, K., Fuchs, J. E., Yarov-Yarovoy, V., Keller, M. A., Golderer, G., Hermetter, A., Werner-Felmayer, G., Hulo, N., Werner, E. R., Biochem. J., 2012, 443, 279–286, © the Biochemical Society.