Figure 1. NIRF imaging of small-sized HT29 colon tumors.

(a) Whole body images of athymic nude mice bearing HT29 tumors after intravenous injection of Cy5.5-P-HA-NPs as a function of time. Arrows indicate the sites of tumors. (b) Intravital fluorescence images of tumor vasculature and Cy5.5-P-HA-NPs. (c) Quantification of the NIRF intensities in the whole bodies and tumor tissues were estimated from the mean fluorescence intensities in 1000 mm² for whole bodies and in 25 mm² for the tumor tissues. Error bars represent the standard deviation (n=3). (d) Ex vivo fluorescence images of organs and tumor excised at 48 h post-injection of NPs (5mg/kg).