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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Science. 2013 Mar 1;339(6123):1088–1092. doi: 10.1126/science.1233321

FIGURE 1. Ifnε signals through the type I IFN receptor but is not induced by TLR ligands nor regulated by IRFs.

FIGURE 1

(A,B) BMMs from WT, Ifnar1−/− and Ifnar2−/− C57BL/6 mice were stimulated with recombinant mouse Ifnα1, Ifnβ or Ifnε (0.1µg/ml) for 3h. (A) Irf7 and (B) 2’5’ oas expression was measured by qRT-PCR. Data are expressed as mean + SEM. of at least three independent experiments. (C) BMMs from C57BL/6 WT mice were treated with a range of TLR ligands or transfected with Poly (I:C) and Poly (dA:dT) for 3h at 37°C. Ifnβ, Il-6 and Ifnε were measured by qRT-PCR. Data are expressed as mean + SEM. of at least three independent experiments. (D) Luciferase reporter plasmids containing Ifnα, Ifnβ, p125, or Ifnε were co-transfected with empty vector or IRF3, IRF7 or IRF5 expression vectors into HEK293 cells. Data are expressed as mean + SEM. All values are means of at least three independent experiments. *P<0.05, **P<0.01, ***P<0.001 (unpaired Student’s t-test).