Skip to main content
. Author manuscript; available in PMC: 2013 Nov 13.
Published in final edited form as: Biochemistry. 2012 Nov 1;51(45):9112–9123. doi: 10.1021/bi300845s

Figure 2.

Figure 2

Chemical cross-linking of the wild-type and mutant USP1/UAF1 complexes using BM(PEG)2. The reaction mixtures were separated on an 8% denaturing SDS-PAGE gel and stained with Coomassie blue. For the wild-type (WT) USP1/UAF1, USP1(S313D)/UAF1, USP1(S67D)/UAF1, and USP1(S67A)/UAF1 complexes, a cross-linked species was observed with a molecular weight close to 212 kDa. No cross-linking product was observed for USP1(S313A)/UAF1 complex.