Table 1.
Enzyme | kcat (s−1) | Km (nM) | kcat/Km (M−1 s−1) | x-fold decrease in kcat/Km |
---|---|---|---|---|
USP1/UAF1 | 0.096 ± 0.005 | 380 ± 130 | 2.5 × 105 | |
USP1(S42A)/UAF1 | 0.081 ± 0.001 | 752 ± 40 | 1.1 × 105 | 2.3 |
USP1(S42D)/UAF1 | 0.089 ± 0.008 | 740 ± 170 | 1.2 × 105 | 2.1 |
USP1(S67A)/UAF1 | 0.070 ± 0.003 | 712 ± 64 | 1.0 × 105 | 2.5 |
USP1(S67D)/UAF1 | 0.068 ± 0.002 | 515 ± 50 | 1.3 × 105 | 1.9 |
USP1(S313A)/UAFI | 0.001 ± 0.0001 | 934 ± 290 | 1.1 × 103 | 227 |
USP1(S313D)/UAF1 | 0.084 ± 0.005 | 332 ± 62 | 2.5 × 105 | 1.0 |