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. Author manuscript; available in PMC: 2013 Nov 13.
Published in final edited form as: Biochemistry. 2012 Nov 1;51(45):9112–9123. doi: 10.1021/bi300845s

Table 1.

Kinetic properties of the wild-type and mutant USP1/UAF1 complexes

Enzyme kcat (s−1) Km (nM) kcat/Km (M−1 s−1) x-fold decrease in
kcat/Km
USP1/UAF1 0.096 ± 0.005 380 ± 130 2.5 × 105
USP1(S42A)/UAF1 0.081 ± 0.001 752 ± 40 1.1 × 105 2.3
USP1(S42D)/UAF1 0.089 ± 0.008 740 ± 170 1.2 × 105 2.1
USP1(S67A)/UAF1 0.070 ± 0.003 712 ± 64 1.0 × 105 2.5
USP1(S67D)/UAF1 0.068 ± 0.002 515 ± 50 1.3 × 105 1.9
USP1(S313A)/UAFI 0.001 ± 0.0001 934 ± 290 1.1 × 103 227
USP1(S313D)/UAF1 0.084 ± 0.005 332 ± 62 2.5 × 105 1.0