Figure 7.

Injury-induced expression of ErbB2 was inhibited in erbin^−/− mice. A, Inability of injury to increase ErbB2 expression in erbin^−/− distal segments. Five days after injury, proximal (P) and distal (D) segments were isolated and subjected to Western blot analysis for ErbB2 and ErbB3. B, Quantitative analysis of data in A. n = 3; **p < 0.01, compared with wild-type controls. ErbB2 and ErbB3 levels were increased to 2.9 ± 0.25- and 1.6 ± 0.22-fold, respectively, above those in proximal segments in wild-type mice (n = 3; p < 0.01). ErbB2 levels in erbin^−/− mice were 2.3 ± 0.46-fold (n = 3; p < 0.05) above those in proximal segments, whereas ErbB3 had no increase. C, mRNA levels of erbB2 and erbB3 in proximal and distal segments of injured sciatic nerves. mRNA was analyzed by quantitative RT-PCR. n = 3; **p < 0.01, compared with wild-type controls.