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. Author manuscript; available in PMC: 2013 Dec 18.
Published in final edited form as: Biochemistry. 2012 Dec 4;51(50):10075–10086. doi: 10.1021/bi3009104

Figure 4.

Figure 4

Steady-state fluorescence change of Ub-AMC and K48-linked IQF diubiquitin binding to C276A USP2. (A) The fluorescence change of 0.5 μM Ub-AMC was plotted against increasing concentrations of USP2 (0-25 μM). (B) The fluorescence change of 25 nM K48-linked IQF diubiquitin was recorded with increasing concentrations of USP2 (0-8 μM). The apparent dissociation constant was determined through nonlinear curve fitting to equation 1 using GraphPad Prism.