Figure 4. Bortezomib induces apoptotic cell death in hypoxic DLD1 but not in HeLa cells.
(A) DLD1 cells were exposed to normoxic or hypoxic conditions for 12h before treatment with DMSO or bortezomib for 24h. Immunoblotting was performed using antibodies specific for cleaved PARP and β-actin (loading control). (B) HeLa cells were exposed to normoxic or hypoxic conditions for 12h before treatment with DMSO or bortezomib for 24h. Staurosporine (STS; 1μM, 4hr) was used as a control. Immunoblotting was performed using antibodies specific for cleaved caspase-3, cleaved PARP and β-actin (loading control).