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. Author manuscript; available in PMC: 2013 Apr 5.
Published in final edited form as: Cancer Res. 2008 Nov 15;68(22):9323–9330. doi: 10.1158/0008-5472.CAN-08-2873

Figure 6. Hypoxic HeLa cells treated with bortezomib undergo necrotic death.

Figure 6

(A) HeLa cells were exposed to normoxic or hypoxic conditions for 12h before treatment with DMSO or bortezomib for 24h. LDH activity was assayed in media off each sample at the indicated doses. Error bars represent s.e. values. (B) Protein was isolated from the media off HeLa cells treated as in A. Freeze/thawed HeLa cells (FT) were used as a control. Immunoblotting was performed using an antibody specific for HMGB1; the Ponceau-stained membrane is shown as a loading control. (C). Model describing the lethal effects of the combination of hypoxia and the proteasome inhibitor bortezomib in DLD1 cells (top) or HeLa cells (bottom). See Discussion for more details.