Figure 1. RNA polymerase II occupancy in islet and non-islet cell lines.

RNA polymerase II occupancy was evaluated in chromatin immunoprecipitation experiments. After fixation with 1% formaldehyde, βTC3, αTC1.6 and NIH3T3 cells were isolated and sonicated as described previously ¹³. DNA/protein complexes were immunoprecipitated by anti-RNA polymerase II antibody (C-terminal domain, Covance). Purified DNA was subjected to quantitative real-time PCR using primers to the indicated genes. Primer sequences and PCR conditions were described previously ¹³. RNA polymerase II occupancy is displayed as percent recovery of the indicated DNA fragment relative to input DNA.