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. Author manuscript; available in PMC: 2013 Apr 5.
Published in final edited form as: Islets. 2009 Nov;1(3):269–272. doi: 10.4161/isl.1.3.9779

Figure 3. -6.5 kilobase pair Set7/9 promoter is sufficient for β cell-line-specific transcription.

Figure 3

A, schematic representation of the Set7/9 promoter (-6584 bp to +316 bp)-LacZ expression construct (in vector pBAT11); B, the expression construct in A or pBAT11 (control vector) was transfected into βTC3 and NIH3T3 cells using Metafectene Pro (Biontex) according to manufacturer's protocol. Total cellular protein was isolated and subjected to immunoblotting using anti-β-galactosidase (Abcam) and anti-actin (clone C4, MP Biomedicals) antibodies, and visualized with the Odyssey system (LICOR Biosciences); C, enzymatic staining for β-galactosidase was performed on βTC3 and NIH3T3 cells transfected with the expression construct shown in A according to a protocol by Open Wet Ware (http://openwetware.org). Blue staining indicates positive β-galactosidase activity.