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. Author manuscript; available in PMC: 2013 Apr 5.
Published in final edited form as: Virology. 2011 May 20;416(0):42–53. doi: 10.1016/j.virol.2011.04.018

Figure 5. Coimmunoprecipitation of UL37HA with GFP tagged N-Terminal and C-Terminal UL37 Truncation Mutants.

Figure 5

(A) A schematic representation of full length, N-terminal and C-terminal UL37 truncation mutants fused to GFP. A tabular summary of results indicate the UL37GFP truncation mutants that bind (+) or do not bind (−) UL37HA in coimmunoprecipitation assays. Molecular weight values were predicted by Statistical Analysis of Protein Sequence accessed through SDSC Biology Workbench. (B) Expression of the UL37 constructs. Vero cells cotransfected with the indicated constructs were harvested approximately 24 h post-transfection and lysed in NP40 lysis buffer. A fraction of the cytoplasmic lysates was analyzed using rabbit antibodies to GFP (αGFP, top panel) and HA (αHA, bottom panel). (C) Coimmunopreciptitation of UL37GFP constructs with UL37HA. Transfected cell lysates were incubated with goat anti-GFP antibody and then bound to protein-G agarose beads. Immunoprecipitated material was analyzed on a SDS-PAGE gel and transferred to nitrocellulose. Proteins were detected by Western blotting using rabbit antibodies to GFP (αGFP, top panel) and HA (αHA, bottom panel).