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. Author manuscript; available in PMC: 2013 Apr 5.
Published in final edited form as: Virology. 2011 May 20;416(0):42–53. doi: 10.1016/j.virol.2011.04.018

Figure 7. Coimmunoprecipitation of UL37HA and UL37.Δ568-809GFP.

Figure 7

(A) A schematic representation of the UL37GFP constructs cotransfected with full length UL37HA construct. A tabular summary of results indicate the UL37GFP truncation mutants that bind (+) or do not bind (−) UL37HA in coimmunoprecipitation assays. Molecular weight values were predicted by Statistical Analysis of Protein Sequence accessed through SDSC Biology Workbench. (B) Expression of UL37HA. Vero cells cotransfected with the indicated constructs were harvested at approximately 24 h post-transfection and lysed in NP40 lysis buffer. A portion of the cytoplasmic lysates was analyzed for protein expression using a rabbit anti-HA antibody. (C) Coimmunoprecipitation of UL37.Δ568-809GFP with UL37HA. Coimmunoprecipitations were performed and analyzed as described in the legend to Fig. 5. Coimmunoprecipitated proteins were detected by Western blotting with rabbit anti-GFP (αGFP, bottom panel) and rabbit anti-HA (αHA, top panel) antibodies.