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. 2012 Dec 11;81(1):195–210. doi: 10.3797/scipharm.1210-19

Tab. 4.

Summary of Stress Degradation Behavior for ASP and ATR

Degradation Type Degradation Condition Sample Type % Degradation observed for ASP % Degradation observed for ATR
Acid Hydrolysis 0.1 N HCl
3 Hrs reflux at 80 °C
ASP alone 24.25
ATR Alone 29.6
ASP:ATR(1:1) Ratio 20.28 38
ATR:ASP(1:7.5)Ratio 32.26 36.40

Alkaline Hydrolysis 0.1 N NaOH
3 Hrs reflux at 80 °C
ASP alone 90
ATR Alone No Degradation
ASP:ATR(1:1) Ratio 90 No Degradation
ATR:ASP(1:7.5)Ratio 93 No Degradation

Neutral Hydrolysis 3Hrs reflux at 80 °C in water ASP alone 19
ATR Alone No Degradation
ASP:ATR(1:1) Ratio 39 6.28
ATR:ASP(1:7.5)Ratio 25.61 27.22

Oxidative Stress 3% H2O2 at room temperature for 7 days ASP alone 54.49
ATR Alone No Degradation
ASP:ATR(1:1) Ratio 89 28
ATR:ASP(1:7.5)Ratio 61 30

Thermal Stress 80 °C for 48 Hrs ASP alone No Degradation
ATR Alone No Degradation
ASP:ATR(1:1) Ratio 39.61 38.57
ATR:ASP(1:7.5)Ratio 15 50

Photolytic Stress Exposed to sunlight for 12 hrs ASP alone No Degradation No Degradation
ATR Alone No Degradation No Degradation
ASP:ATR(1:1) Ratio No Degradation No Degradation
ATR:ASP(1:7.5)Ratio No Degradation No Degradation