Yield (F
v’/F
m’), quantum yield (ФPSII), photochemical quenching coefficient (q
P), non-photochemical quenching (NPQ), and electron transport rate (ETR) in leaf 3 and leaf 4 of an old (released in 1961) and a modern (released in 1997) winter wheat cultivar exposed to elevated CO2 and/or O3 for 21 days in OTCs. Chlorophyll a fluorescence parameters were recorded simultaneously with gas exchange measurement. Leaf chamber environment conditions (i.e. PPFD, temperature, relative humidity, flow rate, and CO2 concentration) were the same as those used for gas exchange measurement: control (CO2, 385±4 ppm+CFA, 4±0.02 ppb O3); elevated CO2 (CO2, 714±16 ppm+CFA, 4±0.02 ppb O3); O3 (ambient CO2, 385±4 ppm+elevated O3, 72±5 ppb O3 for 7h d–1, 9.00–16.00h); and elevated CO2+O3 (elevated CO2, 714±16 ppm+elevated O3, 72±5 ppb for 7h d–1). Overall, elevated CO2 significantly increased (P <0.01) ФPSII, qP, and ETR in the young leaf but decreased NPQ in the matured leaf. Elevated O3 did not alter any light-adapted fluorescence parameter in the young leaf but considerably increased NPQ (P <0.01) in the mature leaf. Results are shown as means±1 standard error (n=8). Means with the same letter were not significantly different.