Fig. 1.

Constructs used in the nodule suppression assay. (A) Site-directed mutants derived from GmRIC1 were created for each residue in the CLE domain as well as several well-conserved residues outside the CLE domain. (B) Chimeric gene constructs were derived from combinations of the GmRIC1 and GmNIC1 signal peptide, variable region, CLE domain, and C-terminal extension. Each construct was named according to the source of each domain (e.g. RRRR for GmRIC1) and was expressed under the 35S promoter in Agrobacterium rhizogenes-induced hairy roots. (This figure is available in colour at JXB online.)