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. 2013 Feb 4;64(6):1521–1536. doi: 10.1093/jxb/ert013

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© The Authors [2013].

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com.

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Fig. 7. — Relative expression levels of six target genes (five ARF genes and one SPL gene) during cotton SE. (A) zhu2_CL4794Contig1 (ARF8) targeted by miR167. (B) zhu2_CL3970Contig2 (ARF6) targeted by miR167. (C) zhu2_CL11135Contig1 (ARF6) targeted by miR167. (D) zhu2_CL3970Contig1 (ARF6) targeted by miR167. (E) zhu2_CL3001Contig1 (ARF6) targeted by miR167. (F) zhu2_gi|164260183|gb|ES799263.1|ES799263 (SPL9) targeted by miR156. The equation ratio=2-ΔCt was applied to calculate the relative expression level using GhUBQ7 as the reference gene; three biological replicates and three technical replicates were performed. 0h, hypocotyls; 6h, 12h, 24h, 48h, 5d, hypocotyls induced for 6, 12, 24, and 48h, and 5 d, respectively; NEC, non-embryonic callus; EC, embryonic callus; GE, globular-stage somatic embryo; TE, torpedo-stage somatic embryo; CE, cotyledon-stage somatic embryo.