Table 1.
Histidine contents and radical-reducing activities of KS-type dehydrin-related peptides. The 50% inhibitory dose (ID50) values were determined by the ROS generation reaction using the Cu–ascorbate system. Ascorbate (300 µM) and CuCl2 (4.6 µM) were used. The results are from four replicates.
| Peptide names | Sequences | Species | AANs | No. of histidines | Histidine contents (%) | ID50 (µM) | ID50×AAN (µM) | Used in Fig. 6 | ||
|---|---|---|---|---|---|---|---|---|---|---|
| Average | SD | Average | SD | |||||||
| AtHIRD11 and domains | ||||||||||
| AtHIRD11 | D1+D2+D3+D4+D5+D6+D7 | Arabidopsis thaliana | 98 | 13 | 13.3 | 0.58 | 0.18 | 56.85 | 17.66 | ✓ |
| D1 | WMAGLINKIGDAL HIGGGNKEG | Arabidopsis thaliana | 22 | 1 | 4.5 | 9.59 | 0.67 | 211.0 | 14.7 | ✓ |
| D2 | WEHKKEEEHKK HVDEHKSGE | Arabidopsis thaliana | 20 | 4 | 20.0 | 1.50 | 0.02 | 30.03 | 0.50 | ✓ |
| D3 | WHKEGIVDKIKDKIHG | Arabidopsis thaliana | 16 | 2 | 12.5 | 3.32 | 0.11 | 53.08 | 1.78 | ✓ |
| D4 | WGEGKSHDGEGKSHDG | Arabidopsis thaliana | 16 | 2 | 12.5 | 3.78 | 0.14 | 60.52 | 2.24 | ✓ |
| D5 | WEKKKKKDKKEKK | Arabidopsis thaliana | 13 | 0 | 0.0 | 195 | 3 | 2535 | 39 | |
| D6 | WHHDDGHH | Arabidopsis thaliana | 8 | 4 | 50.0 | 1.88 | 0.05 | 15.07 | 0.42 | ✓ |
| D7 | WSSSSDSDSD | Arabidopsis thaliana | 10 | 0 | 0.0 | 75.1 | 4.2 | 751.0 | 42.0 | |
| D5+D6+D7 | WEKKKKKDKKEKKHHD DGHHSSSSDSDSD | Arabidopsis thaliana | 29 | 4 | 13.8 | 1.47 | 0.05 | 42.74 | 1.54 | ✓ |
| Modified domains | ||||||||||
| D2H/A | WEAKKEEEAKKAVDEAKSGE | 20 | 0 | 0.0 | 147 | 14 | 2940 | 270 | ||
| D3H/A | WAKEGIVDKIKDKIAG | 16 | 0 | 0.0 | 223 | 64 | 3568 | 1016 | ||
| D4H/A | WGEGKSADGEGKSADG | 16 | 0 | 0.0 | 144 | 32 | 2304 | 509 | ||
| D6H/A | WAADDGAA | 8 | 0 | 0.0 | 196 | 4 | 1568 | 28 | ||
| D6 modified | ||||||||||
| D6 D/A | WHHAAGHH | 8 | 4 | 50.0 | 3.20 | 0.03 | 25.59 | 0.28 | ✓ | |
| D6 D/N | WHHNNGHH | 8 | 4 | 50.0 | 1.85 | 0.10 | 14.78 | 0.82 | ✓ | |
| D6 D/H1 | WHHHDGHH | 8 | 5 | 62.5 | 3.13 | 0.10 | 25.01 | 0.80 | ✓ | |
| D6 D/H2 | WHHHHGHH | 8 | 6 | 75.0 | 2.32 | 0.19 | 18.55 | 1.49 | ✓ | |
| D6 D/H3 | WHHHHHHH | 8 | 7 | 87.5 | 3.14 | 0.41 | 25.14 | 3.26 | ✓ | |
| D6×2 | WHHDDGHHDDGHH | 13 | 6 | 46.2 | 1.35 | 0.13 | 17.52 | 1.63 | ✓ | |
| D6×3 | WHHDDGHHDDGHHDDGHH | 18 | 8 | 44.4 | 0.95 | 0.04 | 17.04 | 0.67 | ✓ | |
| D6 other plants | ||||||||||
| OsD6 | WHGEEGHHHDGH | Oryza sativa | 12 | 5 | 41.7 | 1.69 | 0.20 | 20.25 | 2.37 | ✓ |
| RrD6 | WHEHGHEHGHD | Retama raetam | 11 | 5 | 45.5 | 1.75 | 0.20 | 19.28 | 2.15 | ✓ |
| GmD6 | WHGHDHHGH | Glycine max | 9 | 5 | 55.6 | 2.11 | 0.17 | 19.01 | 1.50 | ✓ |
| SbD6 | WHGEGHDHDGH | Sorghum bicolor | 11 | 4 | 36.4 | 1.85 | 0.25 | 20.35 | 2.78 | ✓ |
| MsD6 | WHGEGHEHGH | Medicago sativa | 10 | 4 | 40.0 | 2.18 | 0.19 | 21.82 | 1.92 | ✓ |
| CpD6 | WHDEHGHDGH | Carica papaya | 10 | 4 | 40.0 | 2.52 | 0.06 | 25.22 | 0.64 | ✓ |
| BdD6 | WHGEGHKKEDGH | Brachypodium distachyon | 12 | 3 | 25.0 | 2.75 | 0.33 | 33.03 | 3.95 | ✓ |
| VvD6 | WHEDGHDHGG | Vaccinium vitis | 10 | 3 | 30.0 | 3.01 | 0.50 | 30.09 | 5.01 | ✓ |
| CmD6 | WHGEGHKHG | Corylus mandshurica | 9 | 3 | 33.3 | 2.40 | 0.12 | 21.64 | 1.09 | ✓ |
| RcD6 | WHEHGH | Ricinus communis | 6 | 3 | 50.0 | 2.87 | 0.11 | 17.19 | 0.68 | ✓ |
| HvD6 | WDGEGHKDDDGH | Hordeum vulgare | 12 | 2 | 16.7 | 2.66 | 0.07 | 31.86 | 0.80 | ✓ |
| PmD6 | WHGEGHDGG | Plantago major | 9 | 2 | 22.2 | 3.18 | 0.18 | 28.62 | 1.65 | ✓ |
| CuD6 | WHEDGHE | Citrus unshiu | 7 | 2 | 28.6 | 4.19 | 0.44 | 29.32 | 3.07 | ✓ |
AAN, amino acid number.