Survival curves for female Aedes aegypti exposed to dengue virus serotype-1 from 0 to 27 days post exposure. A, Effect of temperature fluctuations on the survival of Ae. aegypti females (χ2 = 6.85, degrees of freedom [df] = 2, P = 0.032). B, Effect of infection status on survival (χ2 = 25.17, df = 1, P < 0.0001).