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. 2013 Apr 3;88(4):774–777. doi: 10.4269/ajtmh.12-0594

Figure 1.

Figure 1.

Results of polymerase chain reaction (PCR) and PCR–restriction fragment length polymorphism (RFLP) analysis for three types of clinical specimens (filter paper lesion impressions [FP], lancet scrapings [L], and aspirates [A]) obtained from three skin lesions of a Peruvian patient with cutaneous leishmaniasis. A, kinetoplast DNA PCR; B, mannose phosphate isomerase (mpi) PCR. Upper gel: Leishmania (Viannia) peruviana (Lp) mpi PCR; lower gel: L. (V.) braziliensis [Lb] mpi PCR, indicating amplification of L. (V.) braziliensis mpi target in all 3 lesions (note: only 1 of 3 specimens from lesion 3); C, cysteine proteinase B (cpb) PCR; D, RFLP analysis of cpb PCR products, indicating L. (V.) braziliensis pattern of banding for lesions 1 and 2, and a mixed banding pattern (L. (V.) peruviana or L. (V.) guyanensis [Lg] with filter paper specimen and L. (V.) braziliensis with lancet and aspirate specimens) for lesion 3; E, heat shock protein 70 (hsp70) PCR; F, RFLP analysis of hsp70 PCR products, indicating an L. (V.) braziliensis or L. (V.) peruviana banding pattern for lesions 1 and 2, and an L. (V.) lainsoni banding pattern for lesion 3. Neg = negative. Right lane in each panel is a molecular mass marker.